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BUILD A PROCESS

Build recombinant protein production

Follow a simplified therapeutic-protein production route.

Build a process

Use the up and down buttons to build the sequence, then check your reasoning. Some biological events overlap; follow the simplified pathway described here.

  1. 1
    Purify and assess the product
  2. 2
    Grow selected cells and express the protein
  3. 3
    Introduce the vector into host cells
  4. 4
    Insert the sequence into an expression vector
  5. 5
    Obtain a suitable coding sequence
Read the complete pathway
  1. Obtain a suitable coding sequence. Bacterial expression requires an intron-free sequence and appropriate design.
  2. Insert the sequence into an expression vector. Regulatory sequences support expression.
  3. Introduce the vector into host cells. Cells receiving the construct are identified.
  4. Grow selected cells and express the protein. Controlled culture supports production.
  5. Purify and assess the product. Identity, purity and activity must be checked.

Explain what you observed

Can bacteria always express an unmodified human genomic gene?

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Your study notes

Take the next step

Connect this activity with your lesson notes and chapter practice.

Read a related learning guide →Practise Biotechnology →Open the student notes folder ↗

Concepts and sources

Curriculum connections: 16.3. Selected concepts.

Read the supporting source ↗PMDC curriculum ↗

Questions and explanations are original teaching material; these are not official exam questions.

Keep exploring Biotechnology

Model

PCR: small changes compound

Control cycles, template and amplification efficiency.

Sort & explain

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Separate sequence recognition from antigen recognition.

Sort & explain

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Identify what is supplied to stimulate immunity.